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CUET exam 2023 UG last date, admit card

The Ministry of Education is launching the Common University Entrance Test (CUET exam 2023 UG) for all undergraduate programs offered by Central Universities for the 2023-24 academic year. The purpose of the test is to create a level playing field and equal opportunities for candidates from different regions, particularly those from rural or remote areas. The single entrance examination will allow candidates to have a wider reach and be considered for admission to multiple Central Universities.

CUET Exam 2023 UG Highlights

Name of the exam Common University Entrance Test (CUET)
Exam conducting Authority National Testing Agency
Mode of application Online
Official website cuet.samarth.ac.in
Level of exam National
Total participating universities 90 (44 central universities, 46 other universities)
Mode of exam Computer-based test
Question type Multiple Choice Questions
Exam duration Slot 1 - 45 to 195 minutes Slot 2 - 45 to 225 minutes Slot 3 - To be notified
Medium of exam 13 languages
Exam centres 489 in India, 9 outside India
CUET Exam 2023 UG Highlights

Important deadlines- CUET Exam 2023 UG last date

CUET exam 2023 application form date 09 February 2023
CUET exam 2023 UG last date to apply 12 March 2023
Correction in Particulars 15 March to 18 March 2023 (Up to 11:50 P.M.)
Announcement of the City of Examination 30 April 2023
Downloading of Admit Cards from the NTA website Second week of the May 2023
Date of Examination 21 May 2023 to 31 May 2023 (Reserve dates: 01 to 07 June 2023)
Display of Recorded Responses and Answer Keys To be announced later on the website
Declaration of Result on the NTA website To be announced later on the website
Website(s) www.nta.ac.in, cuet.samarth.ac.in
CUET Exam 2023 UG last date

Scheme of CUET Exam 2023 UG

Section Subjects/ Tests Questions to be
Attempted
Section 1A -
Languages
There are 13 different languages. Any of
these languages may be chosen.
40 questions to be
attempted out of 50 in each language.
Section 1B -
Languages
There are 20 Languages. Any of these
languages may be chosen.
40 questions to be
attempted out of 50 in each language.
Section 2 -
Domain
There are 27 Domains specific subjects
(list given below) being offered under this section. A candidate may choose any subject as desired by the
applicable University/ Universities.
35/40 Questions to be
attempted out of 45/50.
Section 3 -
General Test
For any such undergraduate programme/
programmes being offered by Universities
where a General Test is being used for
admission.
50 Questions to be
attempted out of 60
Scheme of CUET Exam 2023

 

    1. From the above subjects / languages, the candidate can choose maximum of 10 subjects from
      all three Sections.

    1. Examination will be conducted on multiple days in three shifts, depending on the number of
      Candidates and Subject choices.

language-subjects-cuet-exam

 

Language subjects for CUET exam.

Domain subject list for CUET exam 2023 UG

 

List of domain subjects for CUET exam.

Syllabus for CUET Exam 2023 UG

Language syllabus for CUET exam 2023 UG- Language to be tested through Reading Comprehension (based on different types of passages–Factual, Literary and Narrative, and Literary Aptitude and Vocabulary.
Domain Subject syllabus for CUET exam 2023 UG- Download domain subject syllabus
General Test syllabus for CUET exam 2023 UG- General Knowledge, Current Affairs, General Mental Ability, Numerical Ability, Quantitative Reasoning (Simple application of basic mathematical concepts arithmetic/algebra geometry/mensuration/stat),
Logical and Analytical Reasoning
CUET Exam 2023 Syllabus

CUET Application Fees 2023

No. of subjects  General (UR)  OBC (NCL)/EWS  SC/ST/PwBD/Third gender 
Upto 03 Subjects  ₹ 750/-  ₹ 700/-  ₹ 650/- 
Upto 07 Subjects  ₹ 1500/-  ₹ 1400/-  ₹ 1300/- 
Upto 10 Subjects  ₹ 1750/-  ₹ 1650/-  ₹ 1550/- 
* NCL: Non-Creamy Layer 
** EWS: Economically Weaker Section

CUET Exam 2023 UG Admit Card

The National Testing Agency will make the CUET admit cards available on the official website cuet.samarth.ac.in in the second week of May. To obtain the admit card, candidates must log in with their CUET application ID and password. The admit card will contain important information such as the exam center location, date, and time, as well as instructions. It is crucial that candidates print a copy of the admit card and keep it secure until the admission process is finished.

Participating Universities in CUET Exam 2023 UG

A total of 90 universities, made up of 44 central universities and 46 state, deemed, and private universities, are participating in CUET 2023. The scores from the CUET UG exams will be accepted by all of these universities for admission into various CUET programs. These programs include undergraduate courses such as B.A., B.Com, B.Sc, BBA, BCA, and integrated postgraduate courses. The number of seats available for each course in each college will vary. A list of all participating central universities can be found below.

Central Universities Courses offered
Jawaharlal Nehru University B.A. (Hons.) Persian, B.A. (Hons.) Pashto, B.A. (Hons.) Arabic, B.A. (Hons.) Japanese, B.A. (Hons.) Korean, B.A. (Hons.) Chinese, B.A. (Hons.) French, B.A. (Hons.) German, B.A. (Hons.) Spanish, B.Sc.-M.Sc. Integrated Programme in Ayurveda Biology, B.A. (Hons.) Russian,
Mahatma Gandhi Central University  
University of Hyderabad  
University of Delhi  
Hemwati Nandan Bahuguna Garhwal University  
Visva-Bharati University  
University of Allahabad  
Tezpur University  
Central University of Odisha  
Pondicherry University  
English and Foreign Languages University  
Maulana Azad National Urdu University  
Tripura University  
Mizoram University  
National Sanskrit University  
North Eastern Hill University  
Guru Ghasidas Vishwavidyalaya,  
Shri Lal Bahadur Shastri National Sanskrit University  
Sikkim University  
Manipur University  
Dr. Harisingh Gour Vishwavidyalaya  
Mahatma Gandhi Antarrashtriya Hindi Vishwavidyalaya  
The Indira Gandhi National Tribal University  
Central University of Himachal Pradesh  
Central University of Kashmir  
Central University of Jammu  
Central University of Haryana  
Central University of South Bihar  
Central University of Karnataka  
Central University of Rajasthan  
Central University of Jharkhand  
Central University of Kerala  
Central University of Andhra Pradesh  
Central University of Gujarat  
Aligarh Muslim University  
Central University of Tamil Nadu  
List of central universities participating in cuet exam 2023 UG

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Hill station in Almora district gets India’s first grass conservatory

indias first grass conservatory
Representative image.

On Sunday, November 14, 2021, India's first grass conservatory, with a two-acre footprint, opened in Ranikhet, Almora district of Uttarakhand. The conservatory was built in three years by the Uttarakhand Forest Department's research arm with funding from the Central Government's CAMPA scheme.

What is a grass conservatory?

In the protection area, almost 90 different grass species of major scientific, ecological, medicinal, and cultural importance have been grown. There are seven different types of grass species in the protected area, including those used for fragrant, medicinal, feed, ornamental, agricultural, and religious purposes. The project's goal is to raise awareness about grass species' value, encourage their protection, and make field research easier.

What is the need for grass conservatory?

It's also crucial because grasslands are under assault from a variety of sources, and their acreage is declining, putting an entire ecosystem of insects, birds, and mammals that rely on them in jeopardy. Because of their nutritious grain and soil-forming function, grasses are the most economically important of all flowering plants. The endeavour is significant since recent research has shown that grasslands are more effective at 'carbon sequestration' than forest areas.

Thysanolaena, popularly known as Tiger grass or Broom grass, is a valuable fodder grass found in Uttarakhand along steep slopes, ravines, and sandy riverbanks up to an elevation of 2000 metres.

Broom is made from its dry blooming stocks. Because it is a perennial species, it can be utilised as green fodder all year. It also aids in soil erosion prevention on steep hillsides and is employed in land restoration.

Pennisetum Purpureum, often known as Elephant grass or Napier grass, makes a great contour hedgerow as well as bank and pasture feed. Used for firebreaks, windbreaks, and bio-oil, biogas, and charcoal production.

Other worth reading articles for you - 1) State of PhD in India
2) Love-hate relationship among mosquitoes towards light

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Apply now for Kumaun University PhD Entrance Exam 2021: Exam Date, Admit card, Number of seats for Botany, Zoology, Biotechnology

Online application for kumaun university phd entrance exam known as Research degree entrance test (RDET) 2021 are out now.

Online application starts28/October/2021
Last date5/December/2021
Examination FeesRs 2,050
Tentative date of examination19/December/2021
Admit Card DownloadWill be announced later
Examination CentresHaldwani and Nainital
Total Vacancy for Biotechnology2
Total Vacancy for Botany5
Total Vacancy for Zoology2
Research Degree Entrance Test (RDET) 2021

What is the eligibility criteria for Kumaun University PhD Entrance Exam RDET 2021?

1. Candidate should have secured at least fifty five per cent (55%) marks (without rounding off) OR a grade point average equivalent to 55% marks (without rounding off) in the Master's degree examination.

2. For Other Backward Classes (OBC) belonging to Non-Creamy Layer/Scheduled Caste/Scheduled Tribes/Differently Abled Physically (PH)/Visually (VH) candidates, the minimum marks shall be 50% (without rounding off).

3. Note: If the candidates of the reserved category do not furnish documentary evidence/certificate to support their claim they shall be considered under Unreserved Category only.

4. The students who have appeared/appeared in the Post Graduation final year/semester examination of Kumaun University or its affiliated colleges/institutions may also apply provisionally for the PhD entrance Examination.

How to get admission to the Kumaun University PhD programme?

The candidates shall be selected for registration to the PhD degree program through Research Degree Entrance Test (RDET) followed by an Interview.

Can I get Direct admission to Kumaun University PhD, without giving an entrance exam?

1. Yes, one can get direct admission (RDET Exempted i.e. without appearing in RDET entrance exam) if he/she has qualified NETJRF)/Candidates Awarded with Inspire Fellowship/GPAT or equivalent national test, but such candidates shall have to apply by filling the online form of PhD entrance examination along with fees within the due date. These candidates shall have to compulsorily appear for the interview.

2. The final merit will be prepared in accordance with 70% weightage of the marks obtained in examination (NET(JRF)/GPAT) plus 30% weightage of the marks obtained in the interview. In the case of candidates awarded with inspire fellowship, the total marks obtained by the candidate in Post Graduation examination shall be considered to calculate the 70% weightage component.

3. In case the RDET exempted candidate appears in the Research Degree Entrance Test - 2021 the result of RDET-21 written test shall be considered to be final for calculation of Merit. Such candidates shall be not be considered for admission in the PhD program under RDET exempted category.

What is the cutoff marks for Kumaun University PhD entrance exam?

The Qualifying Percentage of Marks in the written Entrance Examination will be:- Unreserved-50%, OBC-45% and SC/ST/VH/PH - 40%.

5. Selection procedure, written exam, and interview

  1. To test the research aptitude of the candidate in related subject a written examination of 100 marks shall be conducted by the University. The total duration of this examination shall be of 02 hrs.
  2. Question paper shall contain 100 objective/multiple-choice type questions and each question shall carry one mark with no negative marking.
  3. RDET will be followed by an interview of 100 marks at the respective department which will be conducted by HOD & Convener of the respective department.
  4. The Qualifying Percentage of Marks in the written Entrance Examination will be:- Unreserved-50%, OBC-45% and SC/ST/VH/PH - 40%.
  5. The candidates who qualify the written examination the final merit will be prepared in accordance with 70% weightage of the marks obtained in written entrance examination plus 30% weightage of the marks obtained in interview.

6. Download Admit Card for Kumaun University PhD 2021 Exam

The download link will be available 7 days before the entrance exam.

7. Free online mock test for kumaun university phd entrance exam RDET 2021

8. Online application for RDET 2021

9. RDET 2021 PhD entrance exam information brochure

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WHO approves first antimalarial drug Mosquirix

First antimalarial drug Mosquirix

The World Health Organisation (WHO) has approved the first antimalarial vaccine RTS, S/AS01 (Mosquirix™) in the world.

The World Health Organization has finally approved the world’s first antimalarial drug. Why is this such a big deal? Well, I’ll tell you why, every two minutes a child dies of malaria and this vaccine can save that child and millions of others. The vaccine is called RTS,S (trade name Mosquirix), it is 40% effective and that too not against all types of malaria parasites. This vaccine targets the parasite that’s most common in Africa- Plasmodium falciparum. It’s a 4 dose vaccine with all doses given between the 5th and the 18th month of a child.

How long did it take for the World Health Organisation to recommend this vaccine? The European Medicines Agency authorized it in 2015 but the WHO sat on it for six years.

How long did the development of an antimalarial drug take? Centuries. Malaria has been claiming lives since the Mesopotamian civilization. In the 20th century alone malaria claimed nearly 300 million lives.

Malaria was not the priority of western countries?

Then why did it take so long to develop a vaccine? Was science not in its favour? Well, the problem was not science, the problem was with lack of political desire. You see research needs money and push but the countries devastated by malaria are the world’s poorest mostly African countries where a lot of families cannot even afford a mosquito net. Malaria was not the priority of the West.

Let us talk some numbers, there are 229 million cases of malaria every year 94% of these cases are reported in Africa. The continent records more than 400,000 malaria deaths every year and most of these victims are children. Outside Africa, less than 90,000 people lose their lives to malaria every year so the world chooses to not care.

Malaria vs Wuhan virus vaccine

In contrast, consider the Wuhan virus it affects rich countries too. In 2020 the western world was suffering, the economy was suffering so companies deep-dived into research and governments poured billions of dollars to create vaccines on priority. The US alone donated $9 billion for COVID development in 2020. What about the malaria vaccine the entire world pulled together $7.3 billion in malaria research from 2007 to 2018 that’s less than a billion dollars every year.

Yes, the malaria parasite is more complicated than the Wuhan virus but the story for the extremely delayed development of a malaria vaccine does not end there. The truth is that a malaria vaccine has never really been a priority. We saw how the covid vaccine smashed all records, we were rolling out doses at speed one couldn’t imagine in the pharmaceutical world. The world made not one not two more than 22 Wuhan virus vaccines in 20 months. Seven of them have already been given emergency use authorization by the World Health Organisation, 124 others are in clinical development stage 194 in preclinical trial and this is not a cooked up story, it’s the data from WHO.

What explains the speed is that the vaccine makers, the approvers, the donors all had the required incentive. These vaccines had a market, a malaria vaccine does not have a market this big. Africa is a continent of 1.3 billion people that’s the population of India. Demand for a malaria vaccine is concentrated in just sub-Saharan Africa. WHO estimates say there could be a requirement of 110 million doses per year by 2036 that’s not a big market not big enough for pharma giants to book their resources. There is also not enough money to be made with the sale of each dose.

GSK partners with Bharat bioetch in the production of first antimalarial drug

GSK or GlaxoSmithKline the company producing the malaria vaccine has said that it will price the jab at 5% above the cost of production. 15 million annual doses will be produced for now and these vaccines will also be made in India. GSK has partnered with India’s Bharat biotech. Another vaccine called R21 is in stage three of clinical trials, it is being developed by Oxford University it will be made by the serum institute of India.

Conclusion

That’s all very well but could all of this have been fast-tracked? had malaria overwhelmed in the hospitals of New York, Italy, Spain or China perhaps the story would have been different and we sincerely hope it doesn’t spread to these parts. That’s not what we’re saying what we’re trying to say is that malaria for Africa is worse than covid. Malaria killed 386,000 Africans in 2019 the Wuhan virus killed 212,000 in the last 18 months.

Because all of this is happening in Africa it never makes it to the headlines but now that we know how fast vaccines can be developed let’s try to not be blinded by profit. The world should apply this urgency to develop vaccines for other diseases too. Vaccines are a lifesaver and we have come a long way, we defeated smallpox, polio but there is still no long term vaccine for tuberculosis, West Nile, Zika, HIV, the malaria vaccine we say is a great start but there's a long way to go.

What is RTS,S or Mosquirix?

RTS,S or Mosquirix is the first malaria vaccine approved by WHO. It will be sold under the trade name mosquirix.

What is the use of Mosquirix?

Mosquirix protects against malaria caused by the parasite Plasmodium falciparum.

Why is the antimalarial drug named RTS,S/AS01?

RTS,S is a scientific name given to this malaria vaccine and represents its composition. The Adjuvant System AS01 is a liposome-based adjuvant that contains two immunostimulants.

The ‘R’ stands for the central repeat region of Plasmodium falciparum circumsporozoite protein (CSP), the ‘T’ for the T-cell epitopes of the CSP, and the ‘S’ for hepatitis B surface antigen (HBsAg).

These are combined in a single fusion protein (‘RTS’) and co-expressed in yeast cells with free HBsAg.

The ‘RTS’ fusion protein and free ‘S’ protein spontaneously assemble in ‘RTS,S’ particles. Thus, in scientific papers, it is usually referred to as ‘RTS,S/AS01’.

Suggested content to read

1. The love-hate relationship of mosquito towards the light

2. State of PhD in India

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What is Dipcovan developed by DRDO

Dipcovan- Covid-19 detection kit

The Defence Institute of Physiology and Allied Sciences (DIPAS), a laboratory of DRDO located in New Delhi has developed a COVID-19 antibody detection kit Dipcovan.
 
It can determine whether or not a person has been exposed to SARS-CoV-2. The kit has a shelf life of 18 months.
 
It has been developed in association with Delhi-based Vanguard Diagnostics Pvt Ltd, for the qualitative detection of IgG antibodies in human serum or plasma, which are developed in response to the coronavirus antigens. The kit will be sold commercially by Vanguard Diagnostics from the first week of June.
 
Dipcovan is a DIPAS-VDx Covid-19 IgG antibody microwell ELISA. It can detect both Spike & Nucleocapsid proteins of the virus with 97% sensitivity and 99% specificity. 
 
It requires 75 minutes for the test to be done, and is expected to be priced at ₹75 per test.
 
 
 
 
 
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Retracted Covid-19 Research Papers

The side-effect of the rat race to publish 

Hitting the Pub Med search tab with the keyword Covid-19 lists a whopping 1,35,105 results for the articles published between 2019-2021.
coronavirus research papers
  Yes, researchers have been working very hard, however, many if not all are seeing this just as an opportunity to publish articles on a topic receiving intense interest from around the globe. The shooting number of research articles is also a consequence of researchers staying at home due to lock down and focusing just on writing rather than conducting research in the labs. Moreover, journals have developed a soft corner for those researchers who are publishing covid-related articles. According to a Nature article Covid related publications were peer-reviewed faster at medical journals while other research articles took longer than usual to get published. Compared with other topics, articles on COVID-19 have been shown to generate more citations. So, just to earn those citation numbers people are minting research articles as if publishing a newspaper column.  As of dated 18/5/2021, 109 papers published on Covid-19 have been retracted while 6 others have earned expressions of concerns. It is still early to conclude whether covid related publications are more prone to retractions than compared to other articles, as many other anomalous articles might still haven't caught the eye of researchers. Leaving aside the lower impact journals, well established reputed journals including Lancet and New England journal of medicine are too amongst those which have received retractions.  Preprints (articles posted online before peer review) of the research articles available on the internet have also increased rapidly during the pandemic. Researchers might know what a preprint is but a common person surfing over the internet is rarely interested in the source and quality of the publication. It is obvious that a preprint that had not undergone proper scrutiny or peer-reviewing may appear trustworthy to someone not from a scientific background.  By the time the preprint undergoes reviewing and is proclaimed as unauthenticated research, the information in it is disseminated to millions of others on social media.  The question that arises is whether permitting early access to data of unclear quality to the few that are knowledgeable enough to save lives justifies exposure of such data to the many that are much less aware of the consequences? Publishing research articles were supposed to involve integrity and honour, but I suppose in the fast-paced world, researchers have become greedy to win the race to grab the maximum number of articles into their basket. There must be countermeasures to tighten the strap on such anomalies. Other articles to read- 1. Electric nose that detect H2S gas 2. State of a phd in India   Data source of retracted papers
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Electric nose that can detect hydrogen sulfide gas

Artificial electric nose made which can detect emissions of hydrogen sulfide gas from sewers

Scientists from the Centre for Nano and Soft Matter Sciences (CeNS) India,in collaboration with King Abdullah University of Science and Technology have developed an electronic nose with biodegradable polymer and monomer that can mimic olfactory receptor neuron, which enables us to detect the smell of various substances.

During the microbial degradation of organic matter under anaerobic conditions in the sewers, hot-springs and during the production of crude-petroleum products, as well as while refining natural gases hydrogen sulphide (H2S) is produced. 

H2S is a poisonous, corrosive, and flammable gas which at concentrations (less than 10 ppm) cause eye irritation and fatigue, and exposure to levels higher than 100 ppm results in severe neurological damage and cardiovascular conditions. 

This artificial nose can protect humans from the harmful impact of this gas while working under such conditions.

The total size of the device is 5 mm × 5 mm, exactly fitting on the tip of the forefinger. 

electric nose that can detect hydrogen sulfide gas
The device can fit the tip of the forefinger. Image credit- https://rsc.li/2PYRR2W

The material of the device

The fabricated sensor consists of a heterostructure consisting of two layers – the top layer a monomer and is realized with a novel chemical tris (keto-hydrazone), which is both porous and contains H2S specific functional groups, and the bottom layer is the active channel layer which plays a key role in altering the current and mobility of charge carriers.

 

artificial electric nose detects hydrogen sulfide gas
The two layers of the device. After exposure to the gas, the chemical reaction confirms the presence of gas. Image credit- https://rsc.li/2PYRR2W

 Thus the synergistic combination helps to pre-concentrate the H2S molecules, initiate an acid-base chemical reaction, and thereby brings a change in the majority carriers (holes) of the channel region in the device.

The capacitance sensor (a sensor that detects nearby objects by their effect on the electrical field created by the sensor) developed by the scientists showed an excellent sensitivity in detecting H2S gas with an experimental limit of detection of around 25 parts per billion. It also has high ambient stability of around 8 months without compromising sensing performance.

Thus, this device can provide early detection of hydrogen sulfide gas present in the particular working area and protect workers from the harmful impact of the gas. Moreover, the devices sensing mechanism can be further modulated for the detection of other gases also.

Source- Research paperVigyaan samachar

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Who got the nobel prize in medicine or physiology 2020?

The year 2020 Nobel prize in Medicine or Physiology has been awarded jointly to 3 scientists Harvey J. Alter and Charles M. Rice from America and Michael Houghton from United Kingdom for their discovery of Hepatitis C virus. 

Harvey J. Alter, Charles M. Rice, and Michael Houghton (From left to right)
 
According to the World Health Organisation, there are 70 million people infected across the globe and 400,000 mortality each year due to the chronic virus. The disease caused by the virus is responsible for liver failure and liver cancer. 

How was the Hepatitis C virus discovered?

Hepatitis B virus was already discovered in the 1960s and was considered to be responsible for blood transmitted hepatitis, therefore, blood used to be tested for the virus before blood transfusions to avoid blood transfusion-related Hepatitis B disease. 

Although the number of patients infected through blood transfusion was reduced post Hepatitis B screening, but a large group of individuals were still contracting the disease after blood transfusions. It meant that apart from Hepatitis B another unidentified causative agent responsible for the disease existed. 

Harvey J. Alter and colleagues showed that blood from these infected individuals transmitted the disease to chimpanzees (only susceptible animal apart from humans). As the disease neither spread from Hepatitis A nor B so it was then called "non-A, non-B" Hepatitis. 

Later almost after a decade, Michael Houghton in 1989 finally identified the unknown causative agent as the Hepatitis C virus. The final question of whether Hepatitis C virus can on its own cause hepatitis was answered by the work of Charles M. Rice. Rice's lab works to understand virus replication and innate immune responses that fight infection. 

Mode of transmission of Hepatitis virus

Both Hepatitis B and C virus are found in the blood of infected individuals, therefore before blood transfusions, one is now checked for the presence of both the viruses. It can also be transmitted by sharing needles, sexual contact, contaminated medical or tattooing equipment's. If a mother is infected with the virus she can also transmit it to her child during birth. It is important to note that Hepatitis A is not transmitted by blood or blood products.

What is the significance of the Hepatitis C virus discovery?

Identification of the causative agent of an infectious disease is the most basic and crucial step in fighting a successful battle against the disease. Due to their discoveries, today we have developed advanced testing kits to identify the Hepatitis C virus and have been able to prevent the blood transmitted hepatitis during blood transfusions.

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How coronavirus testing is being conducted?

The emergence of Coronavirus and Diagnosis

Coronavirus disease (COVID-19) has emerged as that superstar who needs no introduction. Coronam is the Latin term for crown, and coronavirus has the crown-like appearance. As of now, global Covid-19 deaths have exceeded over 8 lakhs with total cases at 25.1 million and still counting. There was only one lab for testing on January 23, 160 labs on March 23 and 1370 labs by August, in India according to the health ministry. 

Previously known as a 2019-novel coronavirus (2019-nCoV) was officially renamed to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by International Committee on Taxonomy of Viruses (ICTV) on February 11, 2020. This name was selected because the virus is genetically related (70% similarity) to the coronavirus responsible for the SARS outbreak of 2002. Diseases are officially named by WHO in the International Classification of Diseases (ICD) while viruses are named by the ICTV.  

Diagnosis of COVID-19 involves multiple phases from sample collection to submitting data of individuals in the ICMR portal.

Sample collection- 

Most commonly, nasal and throat swabs are collected and transported to the nearest testing facility in the Viral transport medium. Each individual is assigned a unique Specimen Referral Form (SRF) ID at the time of sample collection, and details of the individual are recorded.

Coronavirus samples collecting box
Samples are received in the above cooling boxes

Labelling and neutralization- 

Once the samples reach their destined labs, they are allotted lab IDs which are meant to easily distinguish among different samples. Further, neutralization is carried in Biosafety cabinet (BSL-2 and above required), during which virus-cell lysis is done using lysis buffer. This procedure requires maximum preventive measures, as mishandling could result in virus spread. Post neutralization, samples are sent for RNA isolation.

coronavirus samples stored in -80degree
Post neutralization remaining sample volume is stored at -80 ֯ C

RNA isolation- 

Coronaviruses are a large family and its genetic material is a single-stranded positive-sense RNA (+ssRNA); thus, RNA isolation is must to detect its presence in an infected individual. This RNA is then converted to complementary DNA (cDNA), using an enzyme called reverse transcriptase and PCR is done. Internal controls are used as an indicator of perfect nucleic acid extraction, quality of samples, quality of PCR. 

For a quicker screening of individuals pooled sample approach for RNA extraction is being employed. Under this approach, samples from individuals (3 or 5 samples are generally pooled into 1) are mixed into a single tube and RNA extraction proceeds. 

If a pool appears negative for E-gene after RT-PCR (Real-time PCR), then all individuals are considered negative. However, if a pool is detected positive then, RNA extraction is individually done for the pooled samples and tested for E-gene as well as RdRp gene (RNA-dependent RNA polymerase). RdRp is essential for the replication of the virus genetic material once it infects a host. Thus, individuals are diagnosed positive or negative based on the results obtained from two different genes.

Real-time PCR and data analysis-  

In real-time PCR, a positive reaction is determined by the accumulation of a fluorescent signal. Generally, real-time assays undergo 40 cycles of amplification. The cycle at which the fluorescent signal can be detected is termed cycle quantification (Cq) value. 

Some instruments show a cycle threshold (Ct) value instead, which is the number of cycles required for the fluorescent signal to cross the threshold value. Not to be confused, both the Cq and Ct values are technically similar. These values show how many cycles it took to detect a real signal from samples. 

CY5 (cyanine5) is a synthetic dye which detects the internal control and thus confirms the quality of RNA and PCR. If Cq or Ct value for CY5 dye is nil, then the RNA extraction should be repeated. The other dye FAM (fluorescein amidite) is specific for coronavirus specific gene called E-gene. E-gene encodes a small membrane protein (E protein), necessary for the assembly of virions. 

If a person tests positive, then there must be a Cq value for FAM (values greater than 36 are considered as false positives while between 22 to 36 are considered positive), while a person negative for the presence of the virus has no Cq value for FAM. Similarly, FAM is also capable to detect RdRp gene, which is another coronavirus specific gene. Furthermore, positive and negative controls provided with the real-time kit are also run simultaneously to verify the assay.

Real time PCR for coronavirus
Real-time PCR amplification curve showing Cq value of different samples

The relation between Cq value and amount of target gene- 

Cq values are inverse to the amount of the target gene (here E and RdRp gene) expression in samples. Lower Cq values indicate high amounts of target sequence while higher Cq values correspond to weak expression of the target gene.

Other commonly available methods for testing 

Rapid diagnostic tests based on antigen detection

Rapid antigen tests account for about 30% of the total Covid-19 test conducted in India. The rapid test requires sampling similar for a molecular test (real-time PCR), but detects viral proteins (antigens) on the surface of the virus instead of genetic material and also provide results within 10-15 minutes. 

 

However, antigen tests are not as sensitive as molecular tests but are useful due to their quicker output. Rapid antigen tests have been found to provide more false negatives and intermittent false positives. Due to these irregularities in the results, ICMR (Indian Council of Medical Research) has issued an advisory on antigen detection test, individuals who detect negative must be tested successively by RT-PCR to rule out infection, while a positive test should be considered true positive if the symptoms of the patients also, confirm so and does not require confirmation by RT-PCR.

Rapid Antibody detection tests

Antibody detection test is different from the other diagnostic tests, as it identifies people who were infected and have recovered. Unlike RT-PCR or antigen test, antibody detection requires a blood sample.

 

It depends on the detection of antibodies against SARS-CoV2 in a blood sample, which can be easily obtained through a simple finger prick.

 

Two different antibody isotypes, IgM and IgG are detected by this test. If a person has a recent exposure to the virus, then IgM (Immunoglobulin M) antibodies are detected. While the detection of IgG indicates a later stage infection. If both IgG and IgM antibodies are displayed in the test, then the transformation from IgM to IgG humoral response must have been initiated.

 

Antibody response requires several days to develop; thus, this test may not be beneficial in determining the current infection status.

Antibody response against covid-19
Antibody response against Covid-19

The principle behind antibody test- 

IgM antibodies are the first antibodies which are produced initially in response against the virus, therefore indicate a recently initiated infection. While IgG antibodies have a higher affinity to bind with the target antigen (here SARS-CoV2), thus are generated at the later stage of the disease.

 

Technically, an antibody test is similar to the test done for pregnancy detection, which also utilizes a chromatographic immunoassay. 

How long does it require to test covid positive after contacting a positive patient? 

It may take about 3-4 weeks for a person to test positive for an antibody test as it detects the antibodies developed by an individual in their blood. However, an RT-PCR test can detect positive results within 10-14 days after the infection.

Reinfection of Coronavirus

According to the world health organization, it was unclear if being infected meant one can’t be re-infected again. However, recent reports have shown re-infection of patients with COVID-19 from Hongkong, Belgium and Netherland. Thus, precautionary measures are must at these challenging times. 

 

Watch the above video to understand the Rapid antibody test for SARS-CoV-2 
 
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Love-hate relationship among mosquitoes towards light

1. Mosquitoes and their incessant buzz

From vexatious buzz to itchy bites and their exceptional ability to escape from our murderous actions and buzzing around again as if chanting what a loser we were. 

Yes, mosquitoes have been a threat to humans as they are vectors or carriers of deadliest diseases that have affected people across the globe. There are over 3,000 species of mosquitoes in the world and at least 400 of them can be found in India

Before the female mosquito dies, it can produce up to 500 eggs. The only pleasant thing about mosquitoes is that they do not live long, but in the meantime, they contribute to the death of millions of individuals over the world. Yes, millions you read it right, according to World health organization every year mosquitoes result in deaths of over 1 million people around the world.

Fun fact- Only female mosquitoes feed on blood, without which synthesis of yolk and development of eggs is implausible, while male mosquitoes get energy from plant nectar, rotten fruits and honeydew. Male mosquitoes are not able to suck blood from humans due to shorter mandibles. According to research published in the journal Frontiers in physiology, when male mosquitoes were allowed to feed on a blood-soaked cotton roll, they did not survive for too long. Thus concluding that blood is toxic for males.

Below is the list of commonly caused diseases which utilize mosquitoes as a vector.

 

Diseases caused by mosquito

 

2. What research says? 

In the latest research by Baik and colleagues, it has been found that preference of light or darkness by mosquitoes is dependent on the spectrum, time of day, sex and species of mosquitoes. 

Dengue and malaria are the most widely heard diseases associated with mosquitoes, with dengue being caused by day-biting (diurnal) Aedes aegypti. In contrast, malaria is caused by anopheles which targets the vulnerable host at night (nocturnal). Aedes mosquitoes are more active and attracted to light during the day and become inactive and hide in dark locations once they have had their blood meal. In contrast, Anopheles coluzzii, Anopheles gambiae are inactive and specifically avoid ultraviolet (UV) and blue light during the day.

3. Circadian rhythm and mosquito response towards the light

In a simple sense, the circadian rhythm is a 24-hour internal clock within almost every living organism which tells them when to do what irrespective of the external environmental conditions. These clocks are essential in determining the sleeping, feeding patterns and many more functions in all individuals, including humans. 

However, these clocks can also become disrupted if natural external conditions are continuously manipulated, thus ultimately affecting once behavioural aspects. Similarly, in mosquitoes circadian clock functions to help diurnal/nocturnal mosquitoes in differentiating between day or night. While disruption of the clock by continuously exposing the mosquitoes towards artificial light for long hours confuses them and does not allow to distinguish between the day or night. 

Thus, disruption of the circadian clock severely interferes with light-evoked attraction/avoidance behaviour, biting, flight and egg-laying activities in mosquitoes.

4. Why conditional differentiation of mosquito species occurs?

Throughout evolution, different organisms of the species have evolved to differentiate in their abilities to avoid competition for survival. Similarly, different mosquito species might have evolved to occupy unique time-related differences (diurnal and nocturnal), so they can reduce inter-species competition and increase their opportunity of biting, mating and overall existence. It has been found that multiple behavioural changes occur in mosquitoes with respect to time of the day, which includes flight activity, mating, egg-laying, and biting.

5. Why light-based approaches can be substantial to target mosquitoes?

Light-based strategies to control mosquitoes are relatively safer in comparison to toxic pesticides which are harmful to the environment. Moreover, mosquitoes and the disease-causing pathogens have become increasingly resistant towards currently available treatment regimen. 

Thus, an environmentally friendly and innovative method against mosquito control is necessary. With the application of the light-based approach, specific species can be targeted if the timing and the light spectra used is efficiently monitored.

For example, if high-intensity UV light is employed during the day, it will be ineffective against nocturnal mosquitoes. However, the same approach, when utilized appropriately, has produced significant results. A research stated that an exposure of female anopheles mosquito (nocturnal) which causes malaria towards light up to 10 minutes during the night or dusk period can retard its biting and flying ability.

Thus, disruption of the circadian clock by using artificial light may be applied toward species-specific control of harmful diseases like dengue and malaria.

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