Here, we have formulated all the questions from the 2020 PhD biotechnology entrance exam in the form of a quiz, to help all those who will be appearing in this exam in the coming years.

The quiz is available in two parts, each comprising 50 questions. The part-2 is given below.

To solve the other parts click on the link- Kumaun Biotech PhD Part-1

Part-2

Which of the following types of pesticides are least biodegradable?

Organo Chloro compounds

Organo Carbonates

Organic phosphorus compounds

All of the above

PDGF stimulates:

Angiogenesis, guide cell and axon migration

Monocytes proliferation and differentiation

Survival, growth and proliferation of various cell types

Carbohydrate utilization and protein symthesis

Lysol is an:

Antiseptic

Antifungal agent

Sterilent

Disinfectant

Environment tool is an important tool for the development project for which of the following reasons?

1) It supplies to the management with environmental database of
the units
2) It enables the staff and employers to improve their technical
capability, and environmental awareness
3) It safeguards the environment, improves goodwill and public
image of the Company

Only 1

2 and 3

1 and 3

1,2 and 3

Rate of movement of replication fork movement in eukaryotes is approximately .............. times of that in E. coli

2 times

10 times

1/10 times

1/20 times

Water is liquid at room temperature because of-

High melting point of water

Cohesive forces due to hydrogen bonds in water

High boiling point of water

High heat of vaporization of water

When the activity of one gene is suppressed by the activity of a non allelic gene, it is known as

Pseudo-dominance

Hypostasis

Incomplete dominance

Epistasis

Differential staining of bacterial spore is related to:

Albert's staining

Lugol's staining

Moller's staining

Indian ink preparation

Number of antigen binding sites in IgM:

10.

Who of the following scientists pioneered the research on restriction endonucleases?

Meselson

Stahl

Tonegawa

Khorana

1 out of 5

11.

Bioremediation can clean up polluted soils by

Using plants to stimulate soil microbial activity by exuding energy compounds into the rhizosphere

Adding nutrients to stimulate the activity of certain soil bacteria.

Inoculating the soil with certain bacteria.

All of the above

12.

Match each enzyme in column A with the product in column B that it produces:

Column A...................Column B
1. Guanyl cyclase.....a. cAMP
2. Adenyl cyclase......b. DAG and IP₃
3. Phospholipase- C..c. cGMP
4. PIP kinase............d. Phosphalidyl inositol 4,5-biophosphate

1-c, 2-a, 3-b, 4-d

1-d, 2-c, 3-b, 4-a

1-b, 2-c, 3-a, 4-d

1-d, 2-a, 3-d, 4-c

13.

A buffer solution contains ethanoic acid and its conjugate base; the pka of ethanoic acid is 4.74. At what pH does the solution buffer:

6.0

5.0

4.0

3.0

14.

Tears contain which of the following enzymes?

Zymase

Lipase

Lysozyme

Diastase

15.

Best technique for getting pure culture:

Agar slant

Streak plate

Spread plate

Broth culture

16.

Viral replication within cells is inhibited by

IL-4

IL-1

IFNα

TNFα

17.

Somatic embryogenesis is a

sexual mode of embryo formation

asexual mode of embryo formation

Both (A) and (B)

None of the above

18.

Which of the following proteins is NOT a part of Mismatch repair System in E. coli?

Mut H

Mut L

Mut S

Mut M

19.

Average fragment size for a 4-base cutter restriction endonuclease is:

100 bp

250 bp

500 bp

1000 bp

20.

A small amount of lethal mutation is always present in the population due to:

Positive selection

Negative selection

Mutation selection balance

Frequency-dependent selection

2 out of 5

21.

DDT is harmful from the ecological point of view mainly because it undergoes:

Biotransformation

Biomagnification

Bioaccumulation

Biodegradation

22.

What are the pro-inflammatory molecules secreted from

TH₁ Helper T-cells ?

TNF-B, IL-2

TGF-β, II-9

FoxP₃ IL-6

All of these

23.

If there is a solution of 2 M HCN, what concentration of NaCN is needed in order for the pH to be 9.2?

No NaCN needs to be added

24.

The carrying capacity of a BAC vector is approximately upto:

50 kB

100 kB

300 kB

600 kB

25.

A culture medium whose exact composition is not defined is called as:

Simple medium

Complex medium

Defined medium

Natural medium

26.

Match the following :

(a)Chromium...1. Industrial dust, polluted water and foods.
(b) Lead..........2. Industrial fumes and polluted water.
(c) Mercury......3. Polluted water, foods and industrial dust.
(d) Cadmium....4. Industrial fumes, polluted water and food.

(a)..3 (b)..4 (c)..2 (d)..1

(a)..1 (b)..4 (c)..2 (d)..3

(a)..1 (b)..2 (c)..4 (d)..3

(a)..2 (b)..1 (c)..4 (d)..3

27.

Which of the following is/are correct about JAK-STAT pathway ?

P. JAK kinase are tyrosine kinase
Q. STATs are transcription factors and act as substrate for JAK kinase
R. Cytokine receptor activates JAK kinase
S. SH₂ domain in STATs is responsible for dimerization of STATS

P and S

Q, R and S

P,Q, R and S

P, Q, R but not S

28.

Meristematic tissues are used for in-vitro regeneration because :

They are virus free

They are free of fungal infection

They are free of bacterial infection

They lack plasmodesmota

29.

Enzymes that are produced in inactive form in living cells :

Lysozymes

Holoenzyme

Proenzymes

Apoenzyme

30.

In bacteria, Okazaki Aragment size is approximately :

1000 to 2000 N

100 to 200 N

500 to 1000 N

50 to 100 N

3 out of 5

31.

Mt DNA is :

Simple,single stranded, Linear DNA molecule

Simple, double stranded, circular DNA molecule

Simple, double stranded, Linear DNA molecule

Simple, single stranded, circular DNA molecule

32.

Assertion (A) : The radioactive wastes are non-biodegradable. Reason (R) : Certain animals accumulate radioactive elements such as Oyester - Zn-65, Fish - Fe-55, etc

A is true but R is false

A is false but R is true

Both A and R are true and R is correct explanation of A.

Both A and R are true but R is not correct explanation of A.

33.

'cDNA' stands for:

Coiled DNA

Complementary DNA

Circular DNA

Curated DNA

34.

Which of the following is a mismatch ?

Phenotype F2 ratio of 1:4:6:4:1- Polygenic inheritance

Phenotype F2 ratio of 1:2:1 - Co dominance

Phenotype F2 ratio of 9:3:4 -recessive epitasis

Phenotype F2 ratio of 3:1- partial dominance

35.

5' to 3' exonuclease activity is found in which of the following polymerases. in E. coli ?

DNA polymerase I

DNA polymerase II

DNA polymerase III

None of the above

36.

Individual exposed to small pox virus are immune to exposure to same disease again due to :

presence of larger quantities of antibodies

presence of long lived memory cells.

healthy life style

generation of antigen specific macrophages.

37.

Screenable marker i.e. Luciferase was discovered from :

Acquaria victoria

Vibrio harveyi

Photinus pyralis

Both (B) and (C)

38.

Radiations that cause dimerisation of Thymine are :

X- rays

U.V. rays

Infrared

Far-red

39.

The enzymes of the citric acid cycle are located in ?

Mitochondrial matrix

Nucleus

Golgi body

40.

Which of the following is correct statement ?

P- endocrine signalling molecules act on target cell distant from their site of synthesis
Q- paracrine signalling molecules affect target cells only in close proximity.
R- autocrine signalling molecules affect the same cells that release it.
S- synaptic signalling involves neurotransmitters.

P&Q

P & S

P, Q and R

P, Q, R and S

4 out of 5

41.

50 ml of 0.50 M barium hydroxide is required to fully litrate 100 ml solution of sulphuric acid. What is the initial concentration of the acid:

0.25 M

0.5 M

100 M

50 M

42.

Lineweaver -Burk double reciprocal plot is related to :

substrate concentration

enzyme activity

temperature

both (a) and (b)

43.

First synthetic seeds were produced by :

Kitto and Janick (1982)

Mekerise and Bowley(1993)

Redenbaugh and Walker (1990)

Lang and Kohlenboch (1975)

44.

Cell suspension culture is easy to grow and can be easily monitored which is used for :

Synthesizing primary and secondary Metabolites.

Genetic transformation

Producing Vitamins and essential oils

All of the above

45.

A red flowered tall parent plant (P1) was crossed to a true breeding red flowered dwarf plant (P2) and half of the progenies obtained were red and tall and the other red and dwarf. In the next generation, half of all these progenies segregated only for flower colour and the other half segregated only for height. The genotype of the P1 is :

Heterozygous for colour and heterozygous for height.

Heterozygous for colour and homozygous for height

Homozygous for colour and heterozygous for height

Homozygous for colour and homozygous for height

46.

Who of the following scientists purified DNA polymerase I for E.Coli:

Stahl

Kornberg

Brock

Sanger

47.

Howard Temin discovered Reverse transcriptase in :

HIV

HPV

Rous Sarcoma virus

Avian Leukaemia virus

48.

At low blood glucose concentration, brain but not liver will takeup glucose. It is due to the -

blood brain barrier

specificity of glucokinase

low km of glucokinase

low km of hexokinase

49.

Agar is obtained from :

Red algae

Brown algae

Green algae

Blue-green algae

50.

'Alk B' protein is mainly a part of which of the following repair systems in E. coli?

Mismatch repair

Base excision repair

Nucleotide excision repair

Direct repair

5 out of 5

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