Total 100 questions of 100 marks were asked in the entrance exam for PhD Biotechnology Kumaun University. The time duration of the exam is 2 hours and there is no negative marking.

Here, we have formulated all the questions from the 2020 PhD biotechnology entrance exam in the form of a quiz, to help all those who will be appearing in this exam in the coming years. This can give you an idea regarding the type of questions asked and prepare you for a better result.

The quiz is available in two parts, each comprising 50 questions. The part-1 is given below.

To solve the other part click on the link- Kumaun Biotechnology PhD Paper Part-2

Part-1

11.

Which subunit of trimeric G-protein acts as GTPase switch protein subunit ?

βγ

12.

Hardy- Weinberg's principle of "Zero evolution" is based on:

1.Genetic equilibrium
2.High gene frequency
3.No migrations
4.Differential reproduction

3 and 4

1, 2 and 3

1 and 4

2, 3 and 4

13.

How many divisions does a single plant cell undergoes to produce 1000 plant cells?

14.

In E. coli DNA replication, a mistake is made only once for every....... Nucleotides added.

10¹¹ to 10¹²

10⁹ to 10¹⁰

10⁷ to 10⁸

10⁴to 10⁶

15.

Enzyme whose activity can be measured through observation of optical rotation

Isomerase

Invertase

Zymase

Kinase

16.

Which of this statements about hydrogen bond is not true ?

In liquid water, the average water molecule forms hydrogen bonds with three to four other water molecules

Individual hydrogen bonds are much weaker than covalent bonds

Individual hydrogen bonds in liquid water exist for many seconds and sometimes for minutes

Hydrogen bonds account for the anomalously high boiling point of water

17.

Blood agar medium is a type of

Enrichment medium

Differential medium

Selective medium

Enriched medium

18.

Which of the following statements regarding B-cell is true?

1. B-cells produce high affinity auto antibodies
2. Class switching increase affinity of antibodies
3. Maturation of B-cells involves self tolerance
mechanism
4. IgM interacts with both protenaceous and non
protenaceous antigens

2& 4

3& 4

1& 2

1& 4

19.

Following are the Phase I reaction except

Reductive reactions

Sulphide reactions

Oxidative reactions

Hydrolytic reactions

20.

Agrobacterium tumefaciens is a

Gram positive bacterium causing crown-gall disease in monocots

Gram negative bacterium causing crown-gall disease in dicots

Gram positive bacterium causing crown-gall disease in dicots

Gram negative bacterium causing crown-gall disease in monocots

2 out of 5

31.

Coagulase test is used to differentiate:

E.coli

Staphylococcus aureus

Candida albicans

Mycobacterium tuberculosis

32.

After being applied to soil, organic compounds may be destroyed by which of the following processes?

Adsorption to clay or organic matter

Biological degradation

Chemical decomposition

Both (A) and (B)

33.

Gaucher's disease is due to the deficiency of the enzyme:

γ-Galactosidase

β-Galactosidase

α- Galactosidase

Ceramidase

34.

Which of the following is correct regarding the Henderson- Hasselbatch equation?

As the ratio of conjugate base to acid increases, the pH increases.

The pH of a solution is always greater than the pKa of the solution.

The hydrogen ion concentration can never equal the acid dissociation constant.

All are correct

35.

Which of the following conditions will not show inhibitory GPCR signalling?

Mutation in C-terminal end of Receptor

Mutation in GAP

Mutation in GEF

Mutation in the ligand binding domain of Receptor

36.

The net ATP molecules per mole of glucose generated in anaerobic glycolysis:

37.

The pH of a solution of 1 M HCI is

.01

-1

38.

In an individual suffering from viral infection, concentration of IgM and IgG was determined using ELISA. IgM was found in lower quantity. Which of the following conclusion is true based on above observations?

Antigen is encountered for the first time

B-cells are producing hypoactive response.

Major response is provided by memory cells

Major response is provided by Naive B-cells

39.

E. coli on MacConkey Agar medium grow as:

Colourless colonies

Pink coloured colonies

Yellow coloured colonies

Violet coloured colonies

40.

Bacteriophage mediated DNA transfer is known as:

Transformation

Transfection

Transduction

Conjugation

4 out of 5

Kumaun University PhD Entrance Exam Biotechnology

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