Total 100 questions of 100 marks were asked in the entrance exam for PhD Biotechnology Kumaun University. The time duration of the exam is 2 hours and there is no negative marking.

Here, we have formulated all the questions from the 2020 PhD biotechnology entrance exam in the form of a quiz, to help all those who will be appearing in this exam in the coming years. This can give you an idea regarding the type of questions asked and prepare you for a better result.

The quiz is available in two parts, each comprising 50 questions. The part-1 is given below.

To solve the other part click on the link- Kumaun Biotechnology PhD Paper Part-2

Part-1

Identify the correct order in which the extracellular signals are transmitted:

Protein kinase A - CAMP- Adenylate cyclase

Protein kinase A - Adenylate cyclase- CAMP

Adenylate cyclase - CAMP- Protein kinase A

CAMP- Adenylate cyclase- Protein kinase A

Gyanogenesis culturing of ovary and ovule is used for:

in-vitro fertilization

All of the above

Overcoming embryo abortion due to incompatibility barriers

Production of haploid plantlets

A sigmoidal plot of substrate concentration [s] versus reaction velocity (V) may indicate

Michaelis - Menten Kinetics

Co-operative binding

Non-competitive inhibition

Competitive inhibition

For sterilization of fermentation equipment the method followed is:

Heating

Chemicals

All are correct

Radiation

The term Biochemistry was first introduced by :

Carl Neuberg

J Sumner

Svedberg

Karl Erekey

The class of Immunoglobulin that can get transported across epithelial cell is

IgG & IgE

IgG

IgA, IgG & IgM

IgE& IgM

In E. coli which of the following DNA polymerases is mainly responsible for recombination and repair?

DNA polymerase II

DNA polymerase I

DNA polymerase Ill

None is correct

Which of the following chemicals is known to increase the expression of vir-gene?

Carboxy methyl cellulose

Zinc oxide

Acetosyringone

Dextran

If an individual of genotype AaBbCc is test crossed, how many different phenotypes can appear in the progeny?

10.

Xenobiotics are:

Nutrients which kill the gut harmful microbes

Another form of antibiotics

A form of nutrients

Synthetic organic compounds not found in nature

1 out of 5

11.

Which subunit of trimeric G-protein acts as GTPase switch protein subunit ?

βγ

12.

Hardy- Weinberg's principle of "Zero evolution" is based on:

1.Genetic equilibrium
2.High gene frequency
3.No migrations
4.Differential reproduction

3 and 4

1, 2 and 3

1 and 4

2, 3 and 4

13.

How many divisions does a single plant cell undergoes to produce 1000 plant cells?

14.

In E. coli DNA replication, a mistake is made only once for every....... Nucleotides added.

10¹¹ to 10¹²

10⁹ to 10¹⁰

10⁷ to 10⁸

10⁴to 10⁶

15.

Enzyme whose activity can be measured through observation of optical rotation

Isomerase

Invertase

Zymase

Kinase

16.

Which of this statements about hydrogen bond is not true ?

In liquid water, the average water molecule forms hydrogen bonds with three to four other water molecules

Individual hydrogen bonds are much weaker than covalent bonds

Individual hydrogen bonds in liquid water exist for many seconds and sometimes for minutes

Hydrogen bonds account for the anomalously high boiling point of water

17.

Blood agar medium is a type of

Enrichment medium

Differential medium

Selective medium

Enriched medium

18.

Which of the following statements regarding B-cell is true?

1. B-cells produce high affinity auto antibodies
2. Class switching increase affinity of antibodies
3. Maturation of B-cells involves self tolerance
mechanism
4. IgM interacts with both protenaceous and non
protenaceous antigens

2& 4

3& 4

1& 2

1& 4

19.

Following are the Phase I reaction except

Reductive reactions

Sulphide reactions

Oxidative reactions

Hydrolytic reactions

20.

Agrobacterium tumefaciens is a

Gram positive bacterium causing crown-gall disease in monocots

Gram negative bacterium causing crown-gall disease in dicots

Gram positive bacterium causing crown-gall disease in dicots

Gram negative bacterium causing crown-gall disease in monocots

2 out of 5

21.

In the Eukaryotes, which of the following is not a second messenger?

Inositol 1,4,5-triphosphate

Cyclins

1,2-diacyl glycerol

Cyclic AMP

22.

The kinetic effect of purely competitive inhibitor of an enzyme:

decreases V_max without
affecting K_m

decreases K_m without
affecting V_max

increases V_max without
affecting K_m

increases K_m without
affecting V_max

23.

Which among the following is natural cytokinin?

Benzyl amino purine

Zeatin

All are correct

Benzyl adenine

24.

The phenomenon of independent assortment refers to :

Expression at the same stage of development

Association of RNA and a protein implying related function.

Independent location of genes from each other in an interphase cell

Unlinked transmission of genes in crosses resulting from being located on different chromosomes.

25.

In Classical Ames test which of the following bacterial sp. was used?

Pseudomonas sp.

Thermus sp.

Vibrio sp.

Salmonella sp.

26.

Somatic Embryos are encapsulated in a polymer of:

Polyoxyethylene

Polyethylene Glycol (PEG)

Diethyl amino ethyl (DEAE)

All are correct

27.

Molecular Markers are used to construct

Chromosome maps

Cytogenic and physical maps

Both (A) and (B)

Only (B)

28.

Which of the following classes of restriction enzymes target methylated and hydroxymethylated DNA?

Type I

Type II

Type III

Type IV

29.

In Meselson-Stahl experiment what % of first generation DNA molecules are 'hybrid' molecules ?

100%

50%

33.3%

25%

30.

The genetic concept of segregation and recombination are most likely to be associated with

Meiosis and gamete formation

Meiosis and cleavage

Meiosis and mitosis

Meiosis and fertilization

3 out of 5

31.

Coagulase test is used to differentiate:

E.coli

Staphylococcus aureus

Candida albicans

Mycobacterium tuberculosis

32.

After being applied to soil, organic compounds may be destroyed by which of the following processes?

Adsorption to clay or organic matter

Biological degradation

Chemical decomposition

Both (A) and (B)

33.

Gaucher's disease is due to the deficiency of the enzyme:

γ-Galactosidase

β-Galactosidase

α- Galactosidase

Ceramidase

34.

Which of the following is correct regarding the Henderson- Hasselbatch equation?

As the ratio of conjugate base to acid increases, the pH increases.

The pH of a solution is always greater than the pKa of the solution.

The hydrogen ion concentration can never equal the acid dissociation constant.

All are correct

35.

Which of the following conditions will not show inhibitory GPCR signalling?

Mutation in C-terminal end of Receptor

Mutation in GAP

Mutation in GEF

Mutation in the ligand binding domain of Receptor

36.

The net ATP molecules per mole of glucose generated in anaerobic glycolysis:

37.

The pH of a solution of 1 M HCI is

.01

-1

38.

In an individual suffering from viral infection, concentration of IgM and IgG was determined using ELISA. IgM was found in lower quantity. Which of the following conclusion is true based on above observations?

Antigen is encountered for the first time

B-cells are producing hypoactive response.

Major response is provided by memory cells

Major response is provided by Naive B-cells

39.

E. coli on MacConkey Agar medium grow as:

Colourless colonies

Pink coloured colonies

Yellow coloured colonies

Violet coloured colonies

40.

Bacteriophage mediated DNA transfer is known as:

Transformation

Transfection

Transduction

Conjugation

4 out of 5

41.

The major detoxification reactions involved in Phase -I are all except

Oxidation

Acetylation

Reduction

Epoxidation

42.

Which one of the following statements is correct?

TLR -I- Peptidoglycan present in bacterial cell wall

TLR -II- Triacyl lipo-peptide present in mycobacteria

TLR III - It recognizes flagella of bacteria

TLR-IV- It recognizes LPS

43.

As the pka of an acid increases, the acid will be

More stranger

Converted to neutral solution

More weaker

Converted to basic solution

44.

Hormone activating the glycogen synthase activity is:

Glucagon

Insulin

Epinephrine

ACTH

45.

Coomb's Test is used to detect the following in blood samples:

E.coli

Candida albicans

Non-agglutinating antibodies

Sugars

46.

Which one of these is NOT the main effect of polyamines in the tissue culture system?

Promotion of tuber and bulb formation

Adventitious root formation

Promotion of shoot formation

Somatic embryogenesis

47.

What is not true about Histamine?

It is produced on decarboxylation of Histidine

It causes allergy

It lowers blood pressure

Diphenyl hydramine hydrochloride activates histamine

48.

Which of the following vectors are better suited for making genomic DNA library?

_PBluescript

YAC

_PCDNA

_PET

49.

The rolling-circle mechanism of DNA replication is found in :

Gemini virus

Bacteriophage lambda

HPV - 16

All of the above

50.

The genotypic ratio of a monohybrid cross is:

3: 1

1:2:1

2: 1:1

9:3:3:1

5 out of 5

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