GATE Biochemistry paper 2018 quiz

By Kamal Pandey / February 20, 2021 / PhD Entrance

Herein we have provided the GATE 2018 Biochemistry question paper formulated in the form of a quiz. If you wish to solve the complete GATE 2018 life science paper, follow the mentioned link- Click here

To which one of the following classes of enzymes does chymotrypsin belong?

Oxidoreductase

Isomerase

Transferase

Hydrolase

None

Which one of the following conformations of glucose is most stable?

Boat

Planar

Chair

Half Chair

None

Which one of the following amino acids is responsible for the intrinsic fluorescence of proteins?

His

Met

Pro

Trp

None

The glycosylation of the proteins occurs in_____.

plasma membrane

glyoxysomes

Golgi apparatus

lysosomes

None

Which one of the following properties of the myeloma cells is used in the hybridoma technology to generate monoclonal antibody?

lack of hypoxanthine-guanine phosphoribosyl transferase

lack of thymidylate synthase

over-expression of hypoxanthine-guanine phosphoribosyl transferase

over-expression of inosine 5 ́-monophosphate cyclohydrolase

None

The movement of protons through the F₀F₁-ATPase during mitochondrial respiration is
required for ____

the increase in pH of mitochondrial matrix.

decreasing the affinity of ADP to F_oF₁-ATPase.

importing Pi from inter membrane space.

changing the conformation of F_oF₁-ATPase to expel the ATP.

None

The number of NADP⁺ molecules required to completely oxidize one molecule of glucose to CO₂ through pentose phosphate pathway is ____ (correct to integer number).

Measurement of the absorbance of a solution containing NADH in a path length of 1cm cuvette at 340 nm shows the value of 0.31. The molar extinction coefficient of NADH is 6200 M^-1 cm^-1. The concentration of NADH in the solution is ___ μM (correct to integer number).

Among the reagents given below which one of the combination of reagents will NOT break the disulphide bonds in the immunoglobulin molecules?

(P) Reduced glutathione................. (Q) Dithiothritol
(R) Sodium dodecyl sulphate.......... (S) Methionine

P&S

P&R

Q&R

R&S

None

10.

Match the protein elution condition given in Group I with the appropriate chromatography matrices from Group II.

Group I ...............................................................................Group II
P Increasing concentration of sodium chloride ............i Phenyl-Sepharose
Q Increasing concentration of histidine....................... ii Chromatofocusing
R Decreasing concentration of ammonium sulphate....iii DEAE-Sephacryl
S Decreasing concentration of H⁺................................iv Ni-NTA

P-iii; Q-iv; R-i; S-ii

P-i; Q-ii; R-iii; S-iv

P- iv; Q-ii; R-iii; S-i

P-ii; Q-iv; R-i; S-iii

None

1 out of 2

11.

Which one of the following is NOT a neurotransmitter?

Glutamate

Adrenaline

Histidine

Histamine

None

12.

The type-II hypersensitivity reaction is mainly mediated by____.

IgE

IgM

T cells

IgA

None

13.

Which one the following reaction mechanisms drives the conversion of low energy 3- phosphoglyceraldehyde to high energy 1,3-bisphosphoglycerate?

Substrate level phosphorylation

Formation of carboxylate

Oxidation without anhydride bond formation

Oxidation coupled with anhydride bond formation

None

14.

A polymerase reaction is carried out for 10 cycles in a volume of 1 ml with 5 molecules of template DNA. Assuming that the efficiency of the reaction is 100 %, the number of molecules of DNA present in 100 μl at the end of the reaction is ___ (correct to integer number).

15.

An enzyme follows Michaelis-Menten kinetics with substrate S. The fraction of the maximum velocity (V_max) will be observed with the substrate concentration [S] = 4K_m is _____ (correct to one decimal place). (K_m is Michaelis-Menten constant)

16.

The mass spectrum of benzoic acid will generate the fragment as a base peak (100% relative abundance) of m/z (mass to charge ratio) at ____ (correct to integer number).

2 out of 2