1. The fishermen, _______ the flood victims owed their lives, were rewarded by the government.
2. Some students were not involved in the strike. If the above statement is true, which of the following conclusions is/are logically necessary?
1. Some who were involved in the strike were students. 2. No student was involved in the strike. 3. At least one student was involved in the strike. 4. Some who were not involved in the strike were students.
3. The radius as well as the height of a circular cone increases by 10%. The percentage increase in its volume is ______.
4. Five numbers 10, 7, 5, 4 and 2 are to be arranged in a sequence from left to right following the directions given below:
1. No two odd or even numbers are next to each other. 2. The second number from the left is exactly half of the left-most number. 3. The middle number is exactly twice the right-most number. Which is the second number from the right?
5. Until Iran came along, India had never been ________________ in kabaddi.
6. Since the last one year, after a 125 basis point reduction in repo rate by the Reserve Bank of India, banking institutions have been making a demand to reduce interest rates on small saving schemes. Finally, the government announced yesterday a reduction in interest rates on small saving schemes to bring them on par with fixed deposit interest rates. Which one of the following statements can be inferred from the given passage?
7. In a country of 1400 million population, 70% own mobile phones. Among the mobile phone owners, only 294 million access the Internet. Among these Internet users, only half buy goods from e-commerce portals. What is the percentage of these buyers in the country?
8. The nomenclature of Hindustani music has changed over the centuries. Since the medieval period dhrupad styles were identified as baanis. Terms like gayaki and baaj were used to refer to vocal and instrumental styles, respectively. With the institutionalization of music education the term gharana became acceptable. Gharana originally referred to hereditary musicians from a particular lineage, including disciples and grand disciples. Which one of the following pairings is NOT correct?
9. Two trains started at 7AM from the same point. The first train travelled north at a speed of 80km/h and the second train travelled south at a speed of 100 km/h. The time at which they were 540 km apart is ______ AM.
10. “I read somewhere that in ancient times the prestige of a kingdom depended upon the number of taxes that it was able to levy on its people. It was very much like the prestige of a head-hunter in his own community.” Based on the paragraph above, the prestige of a head-hunter depended upon ___________
1 out of 7
11. The Bt toxin gene from Bacillus thuringiensis used to generate genetically modified crops is
12. Which one of the following is used as a pH indicator in animal cell culture medium?
13. Tetracycline inhibits the
14. Which one of the following is a database of protein sequence motifs?
15. Which one of the following enzymes is encoded by human immunodeficiency virus (HIV) genome?
16. DNA synthesis in eukaryotes occurs during which phase of the mitotic cell cycle?
17. Match the human diseases in Group I with the causative agents in Group II.
Group I ................................................Group II P. Amoebiasis ................................1. Leishmania donovani Q. African sleeping sickness............2. Trypanosoma cruzi R. Kala azar ..................................3. Entamoeba histolytica S. Chagas’ disease.........................4. Trypanosoma gambiense
P-3, Q-4, R-2, S-1
P-3, Q-2, R-1, S-4
P-3, Q-4, R-1, S-2
P-4, Q-3, R-1, S-2
None
18. Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?
19. Which of the following processes can increase genetic diversity of bacteria in nature?
P. Conjugation Q. Transformation R. Transduction S. Transfection
20. Which one of the following is NOT a part of the human nonspecific defense system?
2 out of 7
21. A mutation in a gene that codes for a polypeptide results in a variant polypeptide that lacks the last three amino acids. What type of mutation is this?
22. Which one of the following equations represents a one-dimensional wave equation?
23. Which of the following are geometric series?
P. 1, 6, 11, 16, 21, 26, ... Q. 9, 6, 3, 0, -3, -6, ... R. 1, 3, 9, 27, 81, ... S. 4, -8, 16, -32, 64, ...
24. Which one of the following statements is CORRECT for enzyme catalyzed reactions? (ΔG is Gibbs free energy change, Keq is equilibrium constant)
25. Which one of the following can NOT be a limiting substrate if Monod’s growth kinetics is applicable?
26. Which one of the following is the unit of heat transfer coefficient?
27. Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?
28. Which one of the following need NOT be conserved in a biochemical reaction?
29. The number of possible rooted trees in a phylogeny of three species is ________.
30. The median value for the dataset (12, 10, 16, 8, 90, 50, 30, 24) is ___________.
3 out of 7
31. The degree of reduction for acetic acid (C2 H4 O2 ) is __________.
32. The mass of 1 kmol of oxygen molecules is _______ g (rounded off to the nearest integer).
33. Protein concentration of a crude enzyme preparation was 10 mg mL-1 . 10 μL of this sample gave an activity of 5 μmol min-1 under standard assay conditions. The specific activity of this crude enzyme preparation is __________ units mg-1
34. In general, which one of the following statements is NOT CORRECT?
35. For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?
36. Match the organelles in Group I with their functions in Group II.
Group I Group II P. Lysosome 1. Digestion of foreign substances Q. Smooth ER 2. Protein targeting R. Golgi apparatus 3. Lipid synthesis S. Nucleolus 4. Protein synthesis 5. rRNA synthesis
P-1, Q-3, R-2, S-5
P-1, Q-4, R-5, S-3
P-2, Q-5, R-3, S-4
P-1, Q-3, R-4, S-5
None
37. Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?
P. A larger E-value indicates higher sequence similarity Q. E-value < 10-10 indicates sequence homology R. A higher BLAST score indicates higher sequence similarity S. E-value > 1010 indicates sequence homology
38. Which one of the following is coded by the ABO blood group locus in the human genome?
39. Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg2+ concentration Q. pH R. Annealing temperature
40. Group I lists spectroscopic methods and Group II lists biomolecular applications of these methods. Match the methods in Group I with the applications in Group II.
Group I Group II P. Infrared 1. Identification of functional groups Q. Circular Dichroism 2. Determination of secondary structure R. Nuclear Magnetic Resonance 3. Estimation of molecular weight 4. Determination of 3-D structure
4 out of 7
41. The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT?
42. Antibody-producing hybridoma cells are generated by the fusion of a
43. Which of the following statements are CORRECT about the function of fetal bovine serum in animal cell culture?
P. It stimulates cell growth Q. It enhances cell attachment R. It provides hormones and minerals S. It maintains pH at 7.4
44. Which one of the following covalent linkages exists between 7-Methyl guanosine (m7G) and mRNAs?
45. Which one of the following amino acid residues will destabilize an α-helix when inserted in the middle of the helix?
46. What is the solution of the differential equation dy/dx = x/y, with the initial condition, at x =0, y = 1?
47. The Laplace transform of the function f(t) = t2 + 2t + 1 is
48. Which of the following factors can influence the lag phase of a microbial culture in a batch fermentor?
P. Inoculum size Q. Inoculum age R. Medium composition
49. Which one of the following statements is CORRECT about proportional controllers?
50. Match the instruments in Group I with their corresponding measurements in Group II.
Group I .................................Group II P. Manometer ......................1. Agitator speed Q. Rotameter........................2. Pressure difference R. Tachometer......................3. Cell number S. Haemocytometer..............4. Air flow rate
P-4, Q-1, R-2, S-3
P-3, Q-4, R-1, S-2
P-2, Q-4, R-1, S-3
P-2, Q-1, R-4, S-3
None
5 out of 7
51. Which of the following statements is ALWAYS CORRECT about an ideal chemostat?
P. Substrate concentration inside the chemostat is equal to that in the exit stream Q. Optimal dilution rate is lower than critical dilution rate R. Biomass concentration increases with increase in dilution rate S. Cell recirculation facilitates operation beyond critical dilution rate
52. Determine the correctness or otherwise of the following Assertion [a] and the Reason [r] Assertion [a]: It is possible to regenerate a whole plant from a single plant cell. Reason [r]: It is easier to introduce transgenes in to plants than animals.
53. A UV-visible spectrophotometer has a minimum detectable absorbance of 0.02. The minimum concentration of a protein sample that can be measured reliably in this instrument with a cuvette of 1 cm path length is _______ μM. The molar extinction coefficient of the protein is 10,000 L mol-1 cm-1
54. The difference in concentrations of an uncharged solute between two compartments is 1.6-fold. The energy required for active transport of the solute across the membrane separating the two compartments is ____ cal mol-1 (rounded off to the nearest integer). (R= 1.987 cal mol-1 K-1 , T = 37 o C)
55. In pea plants, purple color of flowers is determined by the dominant allele while white color is determined by the recessive allele. A genetic cross between two purple flower- bearing plants results in an offspring with white flowers. The probability that the third offspring from these parents will have purple flowers is _______ (rounded off to 2 decimal places).
56. The molecular mass of a protein is 22 kDa. The size of the cDNA (excluding the untranslated regions) that codes for this protein is _______ kb (rounded off to 1 decimal place).
57. Yeast biomass (C6 H10 O3 N) grown on glucose is described by the stoichiometric equation given below: C6 H12 O6 + 0.48 NH3 + 3 O2 ---------> 0.48 C6 H10 O3 N + 3.12 CO2 + 4.32 H2 O The amount of glucose needed for the production of 50 g L-1 of yeast biomass in a batch reactor with a working volume of 1,00,000 L is ___________ kg (rounded off to the nearest integer).
58. Phenolic wastewater discharged from an industry was treated with Pseudomonas sp. in an aerobic bioreactor. The influent and effluent concentrations of phenol were 10,000 and 10 ppm, respectively. The inlet feed rate of wastewater was 80 L h-1 . The kinetic properties of the organism are as follows: Maximum specific growth rate (μm) = 1 h-1 Saturation constant (KS) = 100 mg L-1 Cell death rate (kd) = 0.01 h-1 Assuming that the bioreactor operates under ‘chemostat’ mode, the working volume required for this process is _________ L (rounded off to the nearest integer).
59. In a cross-flow filtration process, the pressure drop (ΔP) driving the fluid flow is 2 atm, inlet feed pressure (Pi ) is 3 atm and filtrate pressure (Pf ) is equal to atmospheric pressure. The average transmembrane pressure drop (ΔPm ) is __________ atm.
60. An industrial fermentor containing 10,000 L of medium needs to be sterilized. The initial spore concentration in the medium is 106 spores mL-1 . The desired probability of contamination after sterilization is 10-3 . The death rate of spores at 121 o C is 4 min-1 . Assume that there is no cell death during heating and cooling phases. The holding time of the sterilization process is __________ min (rounded off to the nearest integer).
6 out of 7